Protocol

Reconstitution Guide

Step-by-step protocol for safely reconstituting lyophilized peptides for research use.

Required Materials

What you need

Bacteriostatic water (BAC water) — the preferred solvent for most peptides. Sterile syringes and needles (1 mL). Alcohol swabs. The peptide vial. A clean, sterile work surface.

Reconstitution Steps

1

Sanitize the vial tops

Wipe the rubber stopper of both the peptide vial and BAC water vial with a fresh alcohol swab. Allow them to dry for 15–20 seconds before proceeding.

2

Draw the solvent

Using a sterile syringe, draw the desired volume of bacteriostatic water. The volume determines your concentration — e.g., 2 mL into a 5 mg vial gives 2.5 mg/mL.

Tip: 1–2 mL of BAC water per vial is the standard range for most peptides.
3

Inject slowly at an angle

Insert the needle into the peptide vial at an angle so the liquid runs down the inside wall of the vial — do not spray directly onto the lyophilized powder. This prevents foaming and degradation.

4

Gently swirl — never shake

Roll the vial slowly between your fingers until the powder fully dissolves. The solution should become clear. Never vortex or aggressively shake — this can degrade the peptide.

5

Store correctly

Once reconstituted, store the vial in the refrigerator (2–8°C / 35–46°F). Most peptides remain stable for 4–6 weeks after reconstitution. Keep away from light.

Lyophilized (dry) peptides can be stored at –20°C for long-term preservation.

Dosage Calculation

Formula

Concentration (mcg/mL) = Peptide Amount (mcg) ÷ BAC Water Volume (mL)

Example: 5,000 mcg peptide + 2 mL BAC water = 2,500 mcg/mL. A 100 mcg dose = 0.04 mL or 4 IU on a U-100 insulin syringe.

For research purposes only. Not intended for human or veterinary use. All protocols are for in-vitro and laboratory research applications.